HCA Data Explorer

Joint profiling of chromatin accessibility and gene expression in thousands of single cells

Updated September 25, 2023

Here we describe sci-CAR, a combinatorial indexing strategy to jointly profile chromatin accessibility and mRNA in each of thousands of single cells. As a proof-of-concept, we apply sci-CAR to 4,825 cells comprising a time-series of dexamethasone treatment, as well as to 11,233 cells from the mouse kidney.

Junyue CaoUniversity of Washingtoncao1025@uw.edu
Junyue Cao (Experimental Scientist)1
1University of Washington
Teresa Marquez
Beagan Nguy
Parisa Nejad

To reference this project, please use the following link:

https://explore.data.humancellatlas.dev.clevercanary.com/projects/0792db34-8047-4e62-802c-9177c9cd8e28

Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.

1.ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE117nnn/GSE117089/suppl/GSE117089_RAW.tar,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE117nnn/GSE117089/suppl/GSE117089_README.txt,ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE117nnn/GSE117089/suppl/GSE117089_README_filenames_to_SRR.txt
INSDC Project Accessions:GEO Series Accessions:

Atlas

None

Analysis Portals

None

Project Label

JointProfilingChromatinAccessibilityGeneExpression

Species

2 species

Sample Type

2 sample types

Anatomical Entity

4 anatomical entities

Organ Part

Unspecified

Selected Cell Types

4 cell types

Model Organ

3 model organs

Disease Status (Specimen)

2 disease statuses

Disease Status (Donor)

2 disease statuses

Development Stage

4 development stages

Library Construction Method

2 library construction methods

Nucleic Acid Source

single nucleus

Paired End

false, true

File Format

3 file formats

Cell Count Estimate

16.1k

Donor Count

5
fastq.gz2,898 file(s)tar1 file(s)xlsx1 file(s)