Profiling of transcribed cis-regulatory elements in single cells.
Updated July 28, 2022Here we demonstrate single-cell RNA-5′end-sequencing (sc-end5-seq) methods can detect transcribed CREs (tCREs), enabling simultaneous quantification of gene expression and enhancer activities in a single assay at no extra cost. We showed enhancer RNAs can be detected using sc-end5-seq methods with either random or oligo(dT) priming.
Profiling of transcribed cis-regulatory elements in single cells. (Official HCA Publication)
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Atlas
Analysis Portals
NoneProject Label
ProfilingCisRegulatoryElementsSpecies
Homo sapiens
Sample Type
Anatomical Entity
Organ Part
Unspecified
Selected Cell Types
mononuclear cell
Model Organ
Disease Status (Specimen)
normal
Disease Status (Donor)
normal
Development Stage
Library Construction Method
Nucleic Acid Source
Paired End
false, trueAnalysis Protocol
analysis_protocol_bulk_RNA_and_CAGE, analysis_protocol_scATAC-seq, analysis_protocol_scRNA-seq_DMFB_iPSCFile Format
Cell Count Estimate
60.4kDonor Count
3