Low-coverage single-cell mRNA sequencing reveals cellular heterogeneity and activated signaling pathways in developing cerebral cortex.
Updated August 30, 2022The authors captured 301 single cells from 11 populations using microfluidics and analyzing single-cell transcriptomes across downsampled sequencing depths. They demonstrate that shallow single-cell mRNA sequencing (∼50,000 reads per cell) is sufficient for unbiased cell-type classification and biomarker identification in the developing cortex.
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Atlas
Analysis Portals
NoneProject Label
DevelopingCerebralCortexSpecies
Homo sapiens
Sample Type
Anatomical Entity
Organ Part
neocortex
Selected Cell Types
Unspecified
Model Organ
embryo
Disease Status (Specimen)
normal
Disease Status (Donor)
normal
Development Stage
Library Construction Method
Fluidigm C1-based library preparation
Nucleic Acid Source
single cell
Paired End
trueAnalysis Protocol
analysis_protocolFile Format
Cell Count Estimate
301Donor Count
43