HCA Data Explorer

Single-cell RNA sequencing reveals the heterogeneity of liver-resident immune cells in human.

Updated July 19, 2023

The liver plays a critical role in both immune defense and tolerance in the body. The liver-resident immune cells (LrICs) determine the immune properties, but the unique composition and heterogeneity of these cells are incompletely understood. Here, we dissect the diversity of LrICs by a comprehensive transcriptomic profiling using unbiased single-cell RNA-sequencing (scRNA-seq). Overall design: 10x genomics was used to measure CD45+ immune cells in healthy human liver and paired spleen and PBMCs from the same donors.

Zheng ZhangInstitute of Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospitalzhangzheng1975@aliyun.com
Juanjuan Zhao1
Shuye Zhang2
Yang Liu1
Xiaomeng He3
Mengmeng Qu3
Gang Xu1
Hongbo Wang4
Man Huang1
Jing Pan3
Zhenwen Liu4
Zhiwei Li5
Lei Liu1
Zheng Zhang1
1Institute of Hepatology, National Clinical Research Center for Infectious Disease, Shenzhen Third People's Hospital
2Shanghai Public Health Clinical Center and Institute of Biomedical Sciences, Fudan University
3Research Center for Clinical & Translational Medicine, Fifth Medical Center for General Hospital of PLA
4Research Center for Liver Transplantation, Fifth Medical Center for General Hospital of PLA
5Deparment for Liver Transplantation, Shenzhen Third People's Hospital
Ami Day

To reference this project, please use the following link:

https://explore.data.humancellatlas.dev.clevercanary.com/projects/559bb888-7829-41f2-ace5-2c05c7eb81e9
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INSDC Project Accessions:GEO Series Accessions:INSDC Study Accessions:

Atlas

None

Analysis Portals

None

Project Label

HumanLiverImmuneCells_GSE125188

Species

Homo sapiens

Sample Type

specimens

Anatomical Entity

3 anatomical entities

Organ Part

Unspecified

Selected Cell Types

3 cell types

Disease Status (Specimen)

Unspecified

Disease Status (Donor)

Unspecified

Development Stage

human adult stage

Library Construction Method

10X 3' v2 sequencing

Nucleic Acid Source

single cell

Paired End

false

File Format

6 file formats

Cell Count Estimate

207.0k

Donor Count

3
bam9 file(s)fastq.gz18 file(s)h51 file(s)loom12 file(s)txt1 file(s)xlsx1 file(s)