Comprehensive single-cell transcriptome analysis reveals heterogeneity in endometrioid adenocarcinoma tissues
Updated January 23, 2023Single cell transcriptome analysis of a cancer tissue can provide objective assessment of subtype population or the activation of each of various microenvironment component cells. In this study, we applied our newly developed technique of single cell analysis to the myometrial infiltration side (M-side) and the endometrial side (E-side) of a human endometrioid adenocarcinoma with squamous differentiation tissues. We also analyzed spherogenic cultures derived from the same tissue to identify putative regulators of stemness in vivo. Cancer cells in the E-side were highly malignant compared with those in the M-side. Many cells on the E-side were positive for spheroid-specific tumorigenesis-related markers including SOX2. In addition, there were higher numbers of epithelial-to-mesenchymal transition (EMT) cells in the E-side compared with the M-side. This study identified a site containing cells with high malignant potential such as EMT and cancer stem-like cells in cancer tissues. Finally, we demonstrate that established endometrioid adenocarcinoma subtype classifiers were variably expressed across individual cells within a tumor. Thus, such intratumoral heterogeneity may be related to prognostic implications.
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Atlas
Analysis Portals
NoneProject Label
endometrioidAdenocarcinomaSeqSpecies
Homo sapiens
Sample Type
Anatomical Entity
uterus
Organ Part
Selected Cell Types
stem cell
Model Organ
uterus
Disease Status (Specimen)
endometrioid adenocarcinoma
Disease Status (Donor)
endometrioid adenocarcinoma
Development Stage
human adult stage
Library Construction Method
Nx1-seq
Nucleic Acid Source
single cell
Paired End
falseFile Format
Cell Count Estimate
UnspecifiedDonor Count
1