HCA Data Explorer

Comprehensive single-cell transcriptome analysis reveals heterogeneity in endometrioid adenocarcinoma tissues

Updated January 23, 2023

Single cell transcriptome analysis of a cancer tissue can provide objective assessment of subtype population or the activation of each of various microenvironment component cells. In this study, we applied our newly developed technique of single cell analysis to the myometrial infiltration side (M-side) and the endometrial side (E-side) of a human endometrioid adenocarcinoma with squamous differentiation tissues. We also analyzed spherogenic cultures derived from the same tissue to identify putative regulators of stemness in vivo. Cancer cells in the E-side were highly malignant compared with those in the M-side. Many cells on the E-side were positive for spheroid-specific tumorigenesis-related markers including SOX2. In addition, there were higher numbers of epithelial-to-mesenchymal transition (EMT) cells in the E-side compared with the M-side. This study identified a site containing cells with high malignant potential such as EMT and cancer stem-like cells in cancer tissues. Finally, we demonstrate that established endometrioid adenocarcinoma subtype classifiers were variably expressed across individual cells within a tumor. Thus, such intratumoral heterogeneity may be related to prognostic implications.

Shinichi HashimotoKanazawa Universityhashimoto@med.kanazawa-u.ac.jp
Shinichi Hashimoto (Experimental Scientist)1
1Kanazawa University
Rachel Schwartz

To reference this project, please use the following link:

https://explore.data.humancellatlas.dev.clevercanary.com/projects/79b13a2a-9ca1-42a4-97bd-70208a11bea6
None
INSDC Project Accessions:INSDC Study Accessions:

Atlas

None

Analysis Portals

None

Project Label

endometrioidAdenocarcinomaSeq

Species

Homo sapiens

Sample Type

2 sample types

Anatomical Entity

uterus

Organ Part

2 organ parts

Selected Cell Types

stem cell

Model Organ

uterus

Disease Status (Specimen)

endometrioid adenocarcinoma

Disease Status (Donor)

endometrioid adenocarcinoma

Development Stage

human adult stage

Library Construction Method

Nx1-seq

Nucleic Acid Source

single cell

Paired End

false

File Format

2 file formats

Cell Count Estimate

Unspecified

Donor Count

1
fastq.gz8 file(s)xlsx1 file(s)