Single cell RNA-Seq of E18.5 developing mouse kidney and human kidney organoids
Updated August 30, 2022These files represent single cell RNA-Seq data generated on a 10x Chromium genomics platform from three biological replicates from the embryonic day (E)18.5 developing mouse kidney and three biological replicates of iPSC-derived human kidney organoids differentiated according to our published protocol (Takasato et al., Nature Protocols 2016). When aggregated, the mouse data represents >6000 cells that passed our QC, containing most major cell types known to exist in the developing mouse kidney. The aggregated human organoid data contains of >7000 cells that passed our QC and contains populations representing endothelial cells, podocytes, stroma, nephron, and off-target populations with similarity to neurons.
To reference this project, please use the following link:
Supplementary links are provided by contributors and represent items such as additional data which can’t be hosted here; code that was used to analyze this data; or tools and visualizations associated with this specific dataset.
Downloaded and exported data is governed by the HCA Data Release Policy and licensed under the Creative Commons Attribution 4.0 International License (CC BY 4.0). For more information please see our Data Use Agreement.
Atlas
Analysis Portals
NoneProject Label
DevelopingMouseKidneyHumanKidneyOrganoidsSpecies
Sample Type
Anatomical Entity
Organ Part
Selected Cell Types
Unspecified
Model Organ
kidney
Disease Status (Specimen)
normal
Disease Status (Donor)
normal
Development Stage
Library Construction Method
10x 3' v2
Nucleic Acid Source
single cell
Paired End
trueAnalysis Protocol
MergeOptimusLooms_v1.0.0, Optimus_v5.1.1, Optimus_v5.1.2File Format
Cell Count Estimate
14.6kDonor Count
2